Anti-Mouse CD1d [D5] In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse CD1d [D5] In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD1d protein, essential for immune response regulation, making it ideal for research in immunology and related fields. With a mouse IgG1 isotype, it ensures high purity and ultra-low endotoxin levels (<0.002 EU/μg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays.
Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Enhance your research with this reliable and versatile antibody.
CD1d is a glycoprotein expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. It plays a significant role in the immune system by presenting lipid antigens to natural killer T (NKT) cells, thus initiating and regulating immune responses.
Product Name:
Anti-Mouse CD1d (Clone D5) In Vivo Antibody - Ultra Low Endotoxin
Product Code:
IVMB0323
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
D5
Protein:
CD1d
Product Type:
Monoclonal Antibody
Synonyms:
R3G1
Isotype:
IgG2b k
Reactivity:
Mouse
Applications:
In Vivo, IP, WB
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
≤ 0.5 EU/mg as determined by the LAL method
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
In Vivo, IP, WB
Reactivity:
Mouse
Host Species:
Mouse
Specificity:
Clone D5 recognizes an epitope on human CD1d.
Antigen Distribution:
CD1d is expressed on monocytes, DCs, macrophages, and epithelial cells.
Immunogen:
CD1d a1-a2 domains
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
≤ 0.5 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
CD1d antibody, D5, recognizes human CD1d, also known as CD1.1 and Ly-38, a 48 kDa type 1 membrane glycoprotein that belongs to the cluster of differentiation 1 (CD1) family of glycoproteins. CD1d is expressed on epithelial cells and monocyte-derived antigen presenting cells (APCs), including monocytes, DCs, and macrophages1,2. CD1d is an MHC class I-like molecule involved in the presentation of lipid antigens, such as α-galactosylceramide (α-GalCer), to a highly conserved T cell subset, named invariant natural killer T (iNKT) cells or CD1d-restricted T cells1. Following activation, iNKT cells rapidly proliferate and produce large amounts of cytokines and cytolytic mediators3,4.
Protein:
CD1d
Research Area:
Apoptosis, Immunology
Pathogen Testing:
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
