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Anti-Mouse CD19 In Vivo Antibody - Ultra Low Endotoxin

SKU:
IVMB0052
Product Type:
In Vivo Monoclonal Antibody
Clone:
1D3
Protein:
CD19
Isotype:
Rat IgG2a
Reactivity:
Mouse
Research Area:
Immune Checkpoint & Cancer Biology
B Cell Depletion
Endotoxin Level:
Ultra Low Endotoxin
Host Species:
Rat
Applications:
Blocking
CODEX
Depletion
FC
IHC FF
IP
WB
€268 - €3,920
Frequently bought together:

Description

system_update_altTechnical Datasheet

Anti-Mouse CD19 In Vivo Antibody - Ultra Low Endotoxin

Introducing the Anti-Mouse CD19 In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD19 protein, a crucial component in B cell development and function, making it essential for research in immunology and related fields. With a rat IgG2a, lambda isotype, it ensures high purity and ultra-low endotoxin levels (<0.01 EU/μg), ideal for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.

Enhance your research with this reliable and versatile antibody. CD19 is a transmembrane protein expressed on the surface of B cells, playing a significant role in B cell signaling and activation. It is a coreceptor for the B-cell antigen receptor complex and is pivotal in B cell development, differentiation, and response to antigens. This antibody allows for precise targeting and studying of CD19-expressing cells, greatly aiding in the understanding of B cell-associated diseases and therapeutic research.

Product Name:Anti-Mouse CD19 In Vivo Antibody - Ultra Low Endotoxin
Product Code:IVMB0052
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:1D3
Protein:CD19
Product Type:Monoclonal Antibody
Isotype:Rat IgG2a
Reactivity:Mouse
Immunogen:K562 cells expressing the extracellular domain of mouse CD19
Applications:B, Depletion, FC, IHC FF, IP, PhenoCycler®, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0051Anti-Mouse CD19 In Vivo Antibody - Low EndotoxinLow
IVMB0052Anti-Mouse CD19 In Vivo Antibody - Ultra Low EndotoxinUltra Low
Applications:B, CODEX®, Depletion, FC, IHC FF, IP, WB
Recommended Usage:FC
The suggested concentration for this 1D3 antibody for staining cells in flow cytometry is ≤ 1 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
WB
The suggested concentration for this 1D3 antibody for use in western blotting is 1-10 µg/ml.
Reactivity:Mouse
Host Species:Rat
Specificity:Clone 1D3 recognizes an epitope on mouse CD19.
Antigen Distribution:CD19 is expressed in the majority of Pro-B cells to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19.
Immunogen:K562 cells expressing the extracellular domain of mouse CD19
Concentration:≥ 5.0 mg/ml
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD19 is a 95 kD transmembrane glycoprotein and member of the Ig superfamily. The antigen serves as an adaptor protein; drawing cytoplasmic signaling proteins to the membrane. It works via the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. Because of its presence on all B cells, CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for the immunotherapy of lymphoproliferative disorders. Emerging studies indicate that CD19 plays an active role in fueling the growth of these cancers, most notably by stabilizing the concentrations of the MYC oncoprotein, making CD19 an attractive therapeutic target with respect to its downstream signaling.

Technical Datasheet:View
Protein:CD19
Ligand/Receptor:CD21, CD81, Leu-13.
Research Area:Costimulatory Molecules, Immunology
Product Thumbnail
Rat IgG2a Isotype Control
Clone 1-1
Isotype Rat IgG2a
Endotoxin Level Ultra Low Endotoxin
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