Anti-Mouse CD127 (IL-7Rα) [A7R34] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD127 (IL-7R) [A7R34] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD127 protein, a critical component of the interleukin-7 receptor involved in lymphocyte development and homeostasis, making it an ideal choice for research in immunology and related fields. With a rat IgG2a isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays.
Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Elevate your research with this reliable and versatile antibody.
CD127, also known as IL-7R, is a glycoprotein expressed on the surface of immune cells such as T cells, B cells, and natural killer cells. It plays a significant role in the immune system by supporting the survival, differentiation, and proliferation of lymphocytes through interaction with its ligand, interleukin-7 (IL-7).
Product Name:
Anti-Mouse CD127 (IL-7Rα) In Vivo Antibody - Low Endotoxin
B, CyTOF®, FA, FC, IF Staining, IHC FF, In Vivo, IP, WB
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
<0.01 EU/µg as determined by the LAL method
Purity:
>95% monomer by analytical SEC
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
B, CyTOF®, FA, FC, IF Staining, IHC FF, In Vivo, IP, WB
Reactivity:
Mouse
Host Species:
Rat
Specificity:
Clone A7R34 recognizes an epitope on mouse CD127.
Antigen Distribution:
IL-7Rα is expressed on bone marrow lymphoid precursors, pro-B cells, monocytes/macrophages, NK-cells, naïve T cells, memory CD4+ and CD8+ T cells, intestinal colorectal and renal carcinoma cell lines, and keratinocytes.
Immunogen:
IL-7Ra-IgG1 fusion protein
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
<0.01 EU/µg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
>95% monomer by analytical SEC
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
CD127 (IL7R-α) is a 60-90 kD type I cytokine receptor involved in the regulation of lymphopoiesis. The active receptor is an α/gamma chain heterodimer. The common gamma chain, which also associates with the IL-2 receptor, serves mainly to activate signal transduction via the IL7R complex. CD127 also interacts with the Thymic Stromal Lymphopoietin Receptor (TSLPR). CD127 determines signaling events via its association with cytoplasmic signaling molecules and has been reported to be a useful marker for identifying memory and effector T cells.
Protein:
CD127
Function:
T cell and immature B cell proliferation and development
Ligand/Receptor:
IL-7
Research Area:
Immunology
Pathogen Testing:
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
