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Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Ultra Low Endotoxin

SKU:
IVMB0144
Product Type:
In Vivo Monoclonal Antibody
Clone:
55R-170
Protein:
TNFR1
Isotype:
Armenian Hamster IgG
Reactivity:
Mouse
Synonyms:
TNFR-I
TNFRSF1A
P55
P60
Research Area:
Immune Checkpoint & Cancer Biology
Endotoxin Level:
Ultra Low Endotoxin
Host Species:
Armenian Hamster
Applications:
Blocking
FC
IP
WB
€268 - €3,920
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Description

system_update_altTechnical Datasheet

Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Ultra Low Endotoxin

Introducing the Anti-Mouse CD120a - Ultra Low Endotoxin In Vivo Antibody from Assay Genie, a highly specific monoclonal antibody designed for in vivo research applications. This antibody targets CD120a (TNFR1/55R), a key receptor in the TNF-α signaling pathway, making it an exceptional tool for studies in inflammation, apoptosis, and immune response. Classified with mouse IgG isotype, it ensures exceptional purity and ultra-low endotoxin levels (<1.0 EU/mg), thereby meeting rigorous experimental standards and reducing the risk of endotoxin-related interference. This antibody is ideal for applications such as in vivo functional assays, flow cytometry, immunohistochemistry, and other critical research protocols. Available in multiple sizes to accommodate diverse research needs, it is formulated in phosphate-buffered saline for maximum stability and effectiveness.

Enhance the precision and reliability of your research with this robust antibody from Assay Genie. CD120a, also known as TNFR1 (tumor necrosis factor receptor 1), is a receptor found on the surface of a variety of cell types that binds to tumor necrosis factor-alpha (TNF-α). This interaction is crucial for mediating inflammatory and apoptotic pathways. The receptor is implicated in numerous pathological conditions including autoimmune diseases, making it an important target for therapeutic research and intervention.

Product Name:Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Ultra Low Endotoxin
Product Code:IVMB0144
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:55R-170
Protein:TNFR1
Product Type:Monoclonal Antibody
Synonyms:TNFR-I, TNFRSF1A, P55, P60
Isotype:Armenian Hamster IgG
Reactivity:Mouse
Immunogen:Purified Recombinant Mouse TNFR1 (>98%)
Applications:B, FC, IP, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0142Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Low EndotoxinLow
IVMB0144Anti-Mouse CD120a (TNFR1) [55R-170] In Vivo Antibody - Ultra Low EndotoxinUltra Low
Applications:B, FC, IP, WB
Recommended Usage:eLISA
This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
WB To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents and a chromogenic substrate, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.
FC
Reactivity:Mouse
Host Species:Armenian Hamster
Specificity:Clone 55R-170 recognizes mouse CD120a.
Antigen Distribution:CD120a is constitutively expressed in most tissues.
Immunogen:Purified Recombinant Mouse TNFR1 (>98%)
Concentration:≥ 5.0 mg/ml
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Tumor Necrosis Factor Receptor Type I (TNFRI) is a 55kD Type I transmembrane protein. TNFRI is expressed independently of TNFRII at low levels on a wide variety of cell types. TNFRI binds TNF-α and TNF-β (also known as LT-α). The subsequent signaling is known to be important for inducing cytolytic activity, antiviral activity, expression of manganous superoxide dismutase and intercellular adhesion molecule (ICAM), IL-6 mRNA accumulation and NF-ĸB induction. TNFRI carries an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein). Binding of 55R-170 antibody to TNFRI has been shown to block in vitro and in vivo receptor signaling initiated by ligand binding.

Technical Datasheet:View
Protein:TNFR1
Function:Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
Ligand/Receptor:TNF-α, LT-α (TNF-β)
Research Area:Immunology, Innate Immunity
Product Thumbnail
Armenian Hamster IgG Isotype Control
Clone PIP
Isotype Armenian Hamster IgG1
Endotoxin Level Ultra Low Endotoxin
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