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Anti-Mouse CD11a [I21/7] In Vivo Antibody - Ultra Low Endotoxin (IVMB0140)

SKU:
IVMB0140
Antibody Type:
Functional-Grade In Vivo Antibody
Applications:
In Vivo
Disease Area:
Cancer
Clone:
I21/7
Protein:
CD11a
Isotype:
Rat IgG2a
Reactivity:
Mouse
Synonyms:
LFA-1alpha
Endotoxin Level:
Ultra Low Endotoxin
Host Species:
Rat
FC
€359 - €4,859

Description

system_update_altTechnical Datasheet

Anti-Mouse CD11a [I21/7] In Vivo Antibody - Ultra Low Endotoxin

Introducing the Anti-Mouse CD11a [I21/7] In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody meticulously designed for in vivo applications. This antibody targets the CD11a protein, instrumental in leukocyte adhesion and migration, making it ideal for research in immunology, inflammation, and related fields. With a rat IgG2a isotype, it ensures exceptional purity and ultra-low endotoxin levels (<0.01 EU/μg), suitable for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline (PBS) and preservative-free for maximum stability and efficacy.

CD11a, a subunit of LFA-1 (Lymphocyte function-associated antigen 1), is a vital component found on the surface of immune cells such as T-cells, B-cells, macrophages, and neutrophils. It plays a crucial role in the immune system by mediating intercellular adhesion and signaling through interaction with ICAMs (Intercellular Adhesion Molecules), facilitating immune responses and activation pathways. Enhance your research with this high-quality, reliable, and versatile antibody from Assay Genie.

Product Name:Anti-Mouse CD11a [I21/7] In Vivo Antibody - Ultra Low Endotoxin
Product Code:IVMB0140
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:I21/7
Protein:CD11a
Product Type:Monoclonal Antibody
Synonyms:LFA-1α
Isotype:Rat IgG2a
Reactivity:Mouse
Immunogen:Whole T cells Lymphoma BW5147 cells
Applications:FC
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0139Anti-Mouse CD11a [I21/7] In Vivo Antibody - Low EndotoxinLow
IVMB0140Anti-Mouse CD11a [I21/7] In Vivo Antibody - Ultra Low EndotoxinUltra Low
Applications:FC
Recommended Usage:FC
The suggested concentration for clone I21/7 antibody for staining cells in flow cytometry is ≤ 0.5-1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:Mouse
Host Species:Rat
Specificity:Clone I21/7 recognizes an epitope on mouse CD11a.
Antigen Distribution:CD11a is present on thymocytes, blood lymphocytes, bone marrow cells and certain lymphoma and macrophage-like cell lines.
Immunogen:Whole T cells Lymphoma BW5147 cells
Concentration:≥ 5.0 mg/ml
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.

Technical Datasheet:View
Protein:CD11a
Function:Intercellular adhesion
Ligand/Receptor:ICAM-1 (CD54), ICAM-2(CD102), ICAM-3(CD50)
Research Area:Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction
Product Thumbnail
Rat IgG2a Isotype Control
Clone 1-1
Isotype Rat IgG2a
Endotoxin Level Ultra Low Endotoxin
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