MonoMethyl-Histone H4-K20 Polyclonal Antibody (CAB20823)
- SKU:
- CAB20823
- Product Type:
- Antibody
- Antibody Type:
- Secondary Antibody
- Host Species:
- Goat
- Isotype:
- IgG
Description
| Product Name: | MonoMethyl-Histone H4-K20 Polyclonal Antibody |
| SKU: | CAB20823 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat,Other (Wide Range Predicted) |
| Immunogen: | A synthetic monomethylated peptide around K20 of human histone H4 (NP_003539.1). |
| Sequence: | RHRK V |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:100 - 1:500 |
| Synonyms: | H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; H4C16; HIST2H4; HIST2H4A; MonoMethyl-Histone H4-K20 |
| Positive Sample: | HeLa,NIH/3T3,C6,H4 |
| Conjugate: | Unconjugated |
| Cellular Localization: | extracellular exosome, extracellular region, nucleoplasm, nucleus |
| Calculated MW: | 11kDa |
| Observed MW: | 11kDa |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
| Purification Method: | Affinity purification |
| Gene ID: | 8359 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using MonoMethyl-Histone H4-K20 Rabbit pAb (CAB20823) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s. |
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