The Anti-Monomethyl Histone H3 (K79) Antibody (CAB20821) is a valuable tool for researchers studying epigenetics and chromatin modification. This antibody is designed for the detection of monomethyl histone H3 at lysine 79, a specific histone modification involved in gene regulation and chromatin remodeling.Raised in rabbits, this polyclonal antibody is highly reactive with human samples and has been validated for use in various applications, including Western blotting. By specifically binding to monomethyl histone H3 at lysine 79, researchers can accurately detect and analyze the presence of this histone modification in different cell types and tissues.
The study of histone modifications, such as monomethylation of histone H3 at lysine 79, is essential for understanding how gene expression is regulated and how chromatin structure impacts cellular functions. This antibody enables researchers to investigate the role of this specific histone modification in various biological processes, making it a valuable tool for epigenetics research and drug discovery efforts targeting chromatin-modifying enzymes.
Product Name:
MonoMethyl-Histone H3-K79 Polyclonal Antibody
SKU:
CAB20821
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic monomethylated peptide around K79 of human histone H3 (NP_003520.1).
Sequence:
QDFK T
Tested Applications:
WBChIPELISA
Recommended Dilution:
WB,1:100 - 1:500 ChIP,2μg antibody for 5μg-10μg of Chromatin
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H3-K79 Rabbit pAb (CAB20821) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
