The MonoMethyl-Histone H3-K36 Antibody (CAB20566) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for the monomethylated form of Histone H3 at lysine 36 (K36) and is validated for use in various applications including Western blotting and chromatin immunoprecipitation (ChIP).Histone H3 lysine 36 monomethylation is an important histone modification involved in gene regulation, DNA repair, and chromatin organization. Dysregulation of this modification has been linked to various diseases, including cancer and developmental disorders. The Monomethyl Histone H3 (K36) Polyclonal Antibody allows for the precise detection and analysis of this specific histone modification in different cell types, providing valuable insights into the role of epigenetics in various biological processes.
This antibody is validated for use in WB, IF/ICC, ChIP-seq, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
MonoMethyl-Histone H3-K36 Antibody
SKU:
CAB20566
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
GGVK K
Tested Applications:
WBIF/ICCChIP-seqELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H3-K36 Rabbit pAb (CAB20566) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
