MonoMethyl-Histone H3-K18 Monoclonal Antibody (CAB20680)
- SKU:
- CAB20680
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
Product Name: | MonoMethyl-Histone H3-K18 Monoclonal Antibody |
SKU: | CAB20680 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat,Other (Wide Range Predicted) |
Immunogen: | A synthetic monomethylated peptide around K18 of human histone H3 (P68431). |
Sequence: | APRK Q |
Tested Applications: | WB IHC-P IF/ICC IP ChIP ELISA DB CUT&Tag |
Recommended Dilution: | DB,1:500 - 1:1000 WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsIF/ICC,1:50 - 1:200 ChIP,5μg antibody for 5μg-10μg of ChromatinCUT&Tag, 10⁵ cells /1 μg |
Synonyms: | H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; MonoMethyl-Histone H3-K18 |
Positive Sample: | HeLa nuclear extract, NIH/3T3 nuclear extract, C6 nuclear extract |
Conjugate: | Unconjugated |
Cellular Localization: | Chromosome, Nucleus. |
Calculated MW: | 16kDa |
Observed MW: | 17kDa |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method: | Affinity purification |
Gene ID: | 8290/8350 |
Clone Number: | ARC2621 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |