The MERS-CoV Spike RBD Antibody (CAB20565) is a valuable tool for research involving the Middle East Respiratory Syndrome Coronavirus (MERS-CoV), a virus that poses a threat to global public health. This antibody, produced in rabbits, specifically targets the receptor-binding domain (RBD) of the MERS-CoV Spike protein, which is critical for viral entry into host cells.The MERS-CoV Spike RBD Antibody is highly reactive with samples from humans, making it an excellent choice for studies on MERS-CoV infection and transmission. It has been validated for use in Western blot applications, enabling the detection and analysis of the MERS-CoV Spike protein in various cell types.
Understanding the interactions between the MERS-CoV Spike protein and host cells is crucial for developing effective therapeutics and vaccines against MERS-CoV. By studying the role of the Spike RBD in viral entry and infection, researchers can gain insights that may lead to the development of novel antiviral strategies. The MERS-CoV Spike RBD Antibody is a valuable tool for advancing our understanding of this deadly virus and developing interventions to control its spread.
Product Name:
MERS-CoV Spike RBD Rabbit Polyclonal Antibody
SKU:
CAB20565
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 18-725 of coronavirus Spike RBD (YP_009047204.1).
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Purification Method:
Affinity purification
Gene ID:
14254594
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MERS-CoV Spike RBD antibody (CAB20565) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
