The Anti-MELK Antibody (CAB3530) is a highly specific and sensitive tool for studying the expression and function of the maternal embryonic leucine zipper kinase (MELK) protein. MELK is a key player in cell cycle progression and is known to be upregulated in various types of cancer, making it a potential target for cancer therapy.This polyclonal antibody, generated in rabbits, is validated for use in immunohistochemistry and Western blot applications, as well as immunoprecipitation assays. It binds specifically to the MELK protein, enabling accurate detection and quantification in cell lysates and tissue samples.
Researchers in the fields of cancer biology, cell cycle regulation, and drug discovery will find the Anti-MELK Antibody (CAB3530) to be a valuable tool for investigating the role of MELK in tumorigenesis and identifying potential therapeutic targets for cancer treatment. Its high specificity and sensitivity make it an ideal reagent for studying MELK signaling pathways and elucidating its involvement in drug resistance mechanisms.
Product Name:
MELK Rabbit Monoclonal Antibody
SKU:
CAB3530
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MELK (NP_055606.1).
Enables calcium ion binding activity; non-membrane spanning protein tyrosine kinase activity; and protein serine/threonine kinase activity. Involved in apoptotic process; cell population proliferation; and protein autophosphorylation. Located in cell cortex and plasma membrane.
Purification Method:
Affinity purification
Gene ID:
9833
Clone Number:
ARC2032
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MELK Rabbit mAb (CAB3530) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.
