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MEKK3 Rabbit Monoclonal Antibody (CAB3695)

SKU:
CAB3695
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
MAPKKK3
MEKK3
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:MEKK3 Rabbit Monoclonal Antibody
SKU:CAB3695
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEKK3 (Q99759).
Sequence:MDEQ EALN SIMN DLVA LQMN RRHR MPGY ETMK NKDT GHSN RQSD VRIK FEHN GERR IIAF SRPV KYED VEHK VTTV FGQP LDLH YMNN ELSI LLKN QDDL
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:MEKK3; MAPKKK3
Positive Sample:HeLa,NIH/3T3,Jurkat,SH-SY5Y,Mouse spleen
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Cytosol
Calculated MW:71kDa
Observed MW:78kDa

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Purification Method:Affinity purification
Gene ID:4215
Clone Number:ARC2074
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using MEKK3 Rabbit mAb (CAB3695) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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