MEK3/MEK6 Rabbit Monoclonal Antibody (CAB19830)
- SKU:
- CAB19830
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- MAPKK3
- MEK3
- MKK3
- PRKMK3
- SAPKK-2
- SAPKK2
- Research Area:
- Cell Biology
Description
| Product Name: | MEK3/MEK6 Rabbit Monoclonal Antibody |
| SKU: | CAB19830 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 248-347 of human MEK3/MEK6 (P46734). |
| Sequence: | KSDV WSLG ITMI EMAI LRFP YESW GTPF QQLK QVVE EPSP QLPA DRFS PEFV DFTA QCLR KNPA ERMS YLEL MEHP FFTL HKTK KTDI AAFV KEIL GEDS |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | MAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2; MEK3/MEK6 |
| Positive Sample: | HeLa,HT-1080,NIH/3T3,RD,Mouse skeletal muscle,Mouse heart,Rat skeletal muscle,Rat heart |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytosol, Nucleoplasm |
| Calculated MW: | 39,37kDa |
| Observed MW: | 34-40kDa |
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]
| Purification Method: | Affinity purification |
| Gene ID: | 5606/5608 |
| Clone Number: | ARC2356 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of various cell lines, using MEK3/MEK6 Rabbit mAb (CAB19830) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s. |
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