The Anti-MDM2 Antibody (CAB13327) is a valuable tool for researchers studying MDM2, a critical regulator of the p53 tumor suppressor pathway. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By targeting the MDM2 protein, this antibody allows for the detection and analysis of MDM2 expression in various cell types, making it an essential tool for cancer research and drug development.MDM2, also known as mouse double minute 2 homolog, is a key player in the regulation of cell growth and survival, with implications in tumorigenesis and cancer progression.
Its role in the degradation of p53, a potent tumor suppressor, highlights its importance as a therapeutic target for cancer treatment. By investigating the function of MDM2, researchers can gain valuable insights into the molecular mechanisms underlying cancer development and potentially identify novel strategies for anti-cancer therapy.
Product Name:
MDM2 Rabbit Polyclonal Antibody
SKU:
CAB13327
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 200-430 of human MDM2 (NP_001354919.1).
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
Purification Method:
Affinity purification
Gene ID:
4193
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of C2C12, using MDM2 antibody (CAB13327) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
