The MDH1 Polyclonal Antibody (CAB9673) is a valuable tool for researchers studying malate dehydrogenase 1 (MDH1), an enzyme involved in key metabolic processes including the citric acid cycle. Raised in rabbits, this antibody is highly specific for human samples and has been validated for use in Western blot applications. By binding to the MDH1 protein, it allows for accurate detection and analysis in a variety of cell types, making it an essential component for studies in biochemistry and cancer research.MDH1 is essential for converting malate to oxaloacetate, a crucial step in cellular energy production. Dysregulation of MDH1 has been implicated in various diseases, including cancer and metabolic disorders.
By understanding the function and activity of MDH1, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for intervention.Overall, the MDH1 Polyclonal Antibody (CAB9673) offers researchers a reliable tool for investigating the role of MDH1 in cellular metabolism and disease pathology. Its high specificity and sensitivity make it an essential reagent for any laboratory working in the fields of biochemistry, cell biology, or cancer research.
Product Name:
MDH1 Rabbit Monoclonal Antibody
SKU:
CAB9673
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 235-334 of human MDH1 (P40925).
This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.
Purification Method:
Affinity purification
Gene ID:
4190
Clone Number:
ARC1692
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MDH1 Rabbit mAb (CAB9673) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
