The Anti-MDA5 Antibody (CAB2419) is a high-quality research tool designed for the detection and analysis of the melanoma differentiation-associated protein 5 (MDA5). This antibody is produced in rabbits and is highly reactive with human samples, making it ideal for use in various research applications such as Western blotting.MDA5 is a cytoplasmic pattern recognition receptor involved in the detection of viral RNA, playing a crucial role in antiviral immune responses. Dysregulation of MDA5 has been associated with autoimmune diseases such as dermatomyositis and interstitial lung disease.
Understanding the function of MDA5 is essential for uncovering its potential role in disease pathogenesis and developing targeted therapies.By utilizing the Anti-MDA5 Antibody (CAB2419), researchers can effectively study the expression and activity of MDA5 in different cell types and tissues. This antibody provides a valuable tool for investigating the mechanisms underlying viral infections and autoimmune disorders, ultimately contributing to advancements in immunology research.
Product Name:
MDA5 Rabbit Monoclonal Antibody
SKU:
CAB2419
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human MDA5 (Q9BYX4).
IFIH1 encodes MDA5 which is an intracellular sensor of viral RNA that triggers the innate immune response. Sensing RNA length and secondary structure, MDA5 binds dsRNA oligonucleotides with a modified DExD/H-box helicase core and a C-terminal domain, thus leading to a proinflammatory response that includes interferons. It has been shown that Coronaviruses (CoVs) as well as various other virus families, are capable of evading the MDA5-dependent interferon response, thus impeding the activation of the innate immune response to infection. MDA5 has also been shown to play an important role in enhancing natural killer cell function in malaria infection. In addition to its protective role in antiviral responses, MDA5 has been implicated in autoimmune and autoinflammatory diseases such as type 1 diabetes, systemic lupus erythematosus, and Aicardi-Goutieres syndrome
Purification Method:
Affinity purification
Gene ID:
64135
Clone Number:
ARC0760
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using MDA5 Rabbit mAb (CAB2419) at 1:1000 dilution.C6 cells were treated by LPS for 6 hours and TPA for 3 hours of stimulation.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
