Mast Cell Tryptase (TPSB2) Rabbit Monoclonal Antibody (CAB19801)
- SKU:
- CAB19801
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- TPS2
- tryptaseB
- tryptaseC
- Research Area:
- Immunology
Description
| Product Name: | Mast Cell Tryptase (TPSB2) Rabbit Monoclonal Antibody |
| SKU: | CAB19801 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-275 of human Mast Cell Tryptase (TPSB2) (TPSB2) (P20231). |
| Sequence: | GIVG GQEA PRSK WPWQ VSLR VHGP YWMH FCGG SLIH PQWV LTAA HCVG PDVK DLAA LRVQ LREQ HLYY QDQL LPVS RIIV HPQF YTAQ IGAD IALL ELEE PVNV SSHV HTVT LPPA SETF PPGM PCWV TGWG DVDN DERL PPPF PLKQ VKVP IMEN HICD AKYH LGAY TGDD VRIV RDDM LCAG NTRR DSCQ GDSG GPLV CKVN GTWL QAGV VSWG EGCA QPNR PGIY TRVT YYLD WIHH YVPK KP |
| Tested Applications: | WB IHC-P ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 |
| Synonyms: | TPS1; TPS2; TPSB1; TPSB2; Tryptase-2; Mast Cell Tryptase (TPSB2) |
| Positive Sample: | Mouse skin,Rat skin |
| Conjugate: | Unconjugated |
| Cellular Localization: | extracellular space |
| Calculated MW: | 31kDa |
| Observed MW: | 31kDa |
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
| Purification Method: | Affinity purification |
| Gene ID: | 64499 |
| Clone Number: | ARC2328 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using Mast Cell Tryptase (TPSB2) Rabbit mAb (CAB19801) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. |
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