The Anti-Mast Cell Tryptase (TPSB2) Antibody (CAB19801) is a specialized tool for researchers studying mast cell biology and function. This antibody, produced in rabbits, is highly specific and reacts strongly with human mast cell tryptase, a key enzyme involved in mast cell activation and degranulation.Mast cells are crucial in the immune response, playing a role in allergies, asthma, and inflammatory conditions. The TPSB2 antibody is validated for use in various applications, including immunohistochemistry and ELISA, allowing for precise detection and quantification of mast cell tryptase in tissue samples.
By targeting mast cell tryptase, researchers can gain valuable insights into mast cell physiology and the role of these cells in diseases such as allergies, asthma, and inflammatory disorders. The TPSB2 antibody is a valuable tool for those studying mast cell biology and seeking to understand the complex mechanisms underlying mast cell activation and function.
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
Purification Method:
Affinity purification
Gene ID:
64499
Clone Number:
ARC2328
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Mast Cell Tryptase (TPSB2) Rabbit mAb (CAB19801) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
