The LY6G Polyclonal Antibody (CAB20861) is a high-quality antibody developed for reliable detection and analysis of target proteins. Neutrophils are a type of white blood cell involved in the body's immune response to infections and inflammation. This antibody, produced in rabbits, is highly specific to human LY6G and has been validated for use in various research applications such as immunofluorescence and flow cytometry.LY6G is a key marker for identifying and studying neutrophils in both healthy and disease states, making this antibody essential for research in immunology, infectious diseases, and inflammatory conditions.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse samples.
Product Name:
LY6G Polyclonal Antibody
SKU:
CAB20861
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Gr1, Gr-1, Ly-6G
Positive Sample:
Mouse bone marrow
Cellular Localization:
External Side Of Plasma Membrane.
Calculated MW:
14kDa
Observed MW:
20-40kDa
Located in external side of plasma membrane.
Purification Method
Affinity purification
Gene ID
546644
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse bone marrow, using Ly-6G Rabbit pAb (CAB20861) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of paraffin-embedded mouse spleen using Ly-6G Rabbit pAb (CAB20861) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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