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Legumain (LGMN) Rabbit Monoclonal Antibody (CAB6829)

SKU:
CAB6829
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
AEP
LGMN1
PRSC1
Research Area:
Cell Biology
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Legumain (LGMN) Rabbit Monoclonal Antibody
SKU:CAB6829
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 210-302 of human Legumain (LGMN) (Q99538).
Sequence:ANPR ESSY ACYY DEKR STYL GDWY SVNW MEDS DVED LTKE TLHK QYHL VKSH TNTS HVMQ YGNK TIST MKVM QFQG MKRK ASSP VPLP PVTH L
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:AEP; LGMN1; PRSC1; Legumain (LGMN)
Positive Sample:293T,HepG2,MCF7,Mouse brain,Mouse spleen,Mouse kidney
Conjugate:Unconjugated
Cellular Localization:Lysosome.
Calculated MW:49kDa
Observed MW:34kDa/46kDa/56kDa

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.

Purification Method:Affinity purification
Gene ID:5641
Clone Number:ARC1864
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Legumain (LGMN) (LGMN) Rabbit mAb (CAB6829) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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