The Anti-Lactic Acid Histone H4 (K5) Antibody (CAB18829) is a valuable tool for researchers studying histone modifications and their role in gene regulation. This polyclonal antibody, generated in rabbits, is highly specific and sensitive for detecting the histone H4 protein with lysine 5 acetylation. Validated for use in various experimental applications, including Western blot and immunohistochemistry, this antibody allows for accurate detection and visualization of histone H4 (K5) in human samples.Histone modifications, such as acetylation of lysine residues, play a crucial role in the regulation of gene expression and chromatin structure. The acetylation of histone H4 at lysine 5 is associated with active transcription and is linked to processes such as cell proliferation, differentiation, and DNA repair.
By targeting this specific histone modification, researchers can gain insights into the epigenetic mechanisms underlying various biological processes and diseases.The Anti-Lactic Acid Histone H4 (K5) Antibody (CAB18829) is a valuable tool for studying epigenetic regulation and exploring the potential therapeutic implications of histone modifications in diseases such as cancer, developmental disorders, and neurodegenerative conditions. Its high specificity and sensitivity make it a reliable choice for researchers seeking to investigate the intricate mechanisms of gene regulation at the chromatin level.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from MCF7 cells, using Lactic acid -Histone H4-K5 Rabbit pAb (CAB18829) at 1:1000 dilution.MCF7 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
