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Lactic acid-Histone H3-K27 Rabbit Polyclonal Antibody (CAB18825)

SKU:
CAB18825
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
HIST1H3A
H3/A
H3FA
histone H3.1
Research Area:
Epigenetics and Nuclear Signaling
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Lactic acid-Histone H3-K27 Rabbit Polyclonal Antibody
SKU:CAB18825
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:A synthetic lactylated peptide around K27 of human Histone H3 (NP_003520.1).
Sequence:AARK S
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Lactic acid-Histone H3-K27
Positive Sample:C6
Cellular Localization:Chromosome, Nucleus.
Calculated MW:15kDa
Observed MW:17kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Purification Method:Affinity purification
Gene ID:8290/8350
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from C6 cells, using Lactic acid-Histone H3-K27 Rabbit pAb (CAB18825) at 1:1000 dilution.C6 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.

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