The Anti-Lactic Acid Histone H3 (K18) Antibody (CAB18807) offered by AssayGenie is a valuable tool for research involving histone H3 modifications, specifically lysine 18 methylation. This polyclonal antibody, raised in rabbits, is highly specific and reactive with human samples, making it an ideal choice for various experimental applications such as Western blotting and immunofluorescence.Histone H3 lysine 18 methylation has been implicated in various biological processes, including gene regulation, DNA repair, and chromatin remodeling. The Anti-Lactic Acid Histone H3 (K18) Antibody allows for the detection and analysis of this specific histone modification, providing researchers with valuable information on its role in cellular functions and disease development.
Whether studying epigenetic regulation in cancer, development, or other disease states, this antibody is a reliable tool for understanding the complex mechanisms involving histone modifications. Its high specificity and sensitivity make it a trusted choice for researchers in the fields of molecular biology, genetics, and oncology. Unlock the potential of histone H3 lysine 18 methylation research with the Anti-Lactic Acid Histone H3 (K18) Antibody from AssayGenie.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from MCF7 cells, using Lactic acid-Histone H3-K18 Rabbit pAb (CAB18807) at 1:500 dilution.MCF7 cells were treated by Sodium lactate(100mM) for 24h.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5min.
