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Western blot analysis of extracts of various cell lines, using Lactate Dehydrogenase C antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Key Features

Lactate Dehydrogenase C Monoclonal Antibody (CAB20866)

Datasheet MSDS
SKU CAB20866
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human
Host Species Rabbit
Synonyms CT32, LDH3, LDHX, Lactate Dehydrogenase C
€131 - €457

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Description

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Lactate Dehydrogenase C Monoclonal Antibody (CAB20866)

The Lactate Dehydrogenase C Monoclonal Antibody (CAB20866) is a high-quality antibody developed for reliable detection and analysis of target proteins. LDH-C is crucial for the conversion of lactate to pyruvate during anaerobic respiration, making it a key player in metabolic processes.This polyclonal antibody, raised in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By binding specifically to the LDH-C protein, researchers can accurately detect and analyze this enzyme in a variety of cell types.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:Lactate Dehydrogenase C Monoclonal Antibody
SKU:CAB20866
Size:20μL, 100μL
Reactivity:Human
Clone Number:ARC2796
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MSTV KEQL IEKL IEDD ENSQ CKIT IVGT GAVG MACA ISIL LKDL ADEL ALVD VALD KLKG EMMD LQHG SLFF STSK ITSG KDYS VSAN SRIV IVTA GARQ
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CT32, LDH3, LDHX, Lactate Dehydrogenase C
Positive Sample:Human testis
Cellular Localization:Cytoplasm.
Calculated MW:36kDa
Observed MW:36kDa

Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region.

Purification MethodAffinity purification
Gene ID3948
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Lactate Dehydrogenase C Rabbit mAb (CAB20866) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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