The Kinesin Light Chain 1 (KLC1) Polyclonal Antibody (CAB19249) is a valuable tool for researchers studying kinesin light chain proteins, which play a key role in intracellular transport and cell division processes. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the KLC1 protein, this antibody enables precise detection and analysis of KLC1 expression in a variety of cell types, making it an essential reagent for studies in cell biology and cancer research.
KLC1 is a crucial component of the kinesin motor protein complex, which is responsible for transporting cargo along microtubules within cells. Dysregulation of kinesin proteins has been linked to various diseases, including cancer and neurodegenerative disorders. By studying KLC1 expression and activity, researchers can gain valuable insights into the molecular mechanisms underlying these conditions, ultimately paving the way for the development of novel therapeutic interventions targeting kinesin-mediated processes.
Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined.
Purification Method:
Affinity purification
Gene ID:
3831
Clone Number:
ARC2409
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Kinesin light chain 1 (Kinesin light chain 1 (KLC1)) antibody (CAB19249) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
