The Anti-Kaiso (ZBTB33) Antibody (CAB3770) is a valuable tool for researchers studying the Kaiso zinc finger transcription factor. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting and immunohistochemistry.Kaiso, also known as ZBTB33, is a transcription factor involved in regulating gene expression and is implicated in various cellular processes, including cell proliferation, differentiation, and apoptosis.
Its role in cancer development, metastasis, and tumor progression makes it an important target for cancer research.By specifically binding to the Kaiso protein, this antibody enables the detection and analysis of Kaiso in different cell types, providing researchers with a powerful tool to investigate its function and role in cancer biology. Understanding the mechanisms by which Kaiso operates can provide insights into potential therapeutic targets for cancer treatment and management.
Product Name:
Kaiso/ZBTB33 Rabbit Monoclonal Antibody
SKU:
CAB3770
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 573-672 of human Kaiso/ZBTB33 (Q86T24).
This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.
Purification Method:
Affinity purification
Gene ID:
10009
Clone Number:
ARC2086
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Kaiso/ZBTB33 Rabbit mAb (CAB3770) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
