[KO Validated] ITCH Rabbit Monoclonal Antibody (CAB8624)
- SKU:
- CAB8624
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- ADMFD
- AIF4
- AIP4
- NAPP1
Frequently bought together:
Description
| Product Name: | [KO Validated] ITCH Rabbit Monoclonal Antibody |
| SKU: | CAB8624 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ITCH (Q96J02). |
| Sequence: | MSDS GSQL GSMG SLTM KSQL QITV ISAK LKEN KKNW FGPS PYVE VTVD GQSK KTEK CNNT NSPK WKQP LTVI VTPV SKLH FRVW SHQT LKSD VLLG TAAL |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | AIF4; AIP4; ADMFD; NAPP1; CH |
| Positive Sample: | HeLa,293T,Raji,NIH/3T3,C6,Mouse lung |
| Conjugate: | Unconjugated |
| Cellular Localization: | cell cortex, cytoplasm, cytoplasmic vesicle, cytosol, extracellular exosome, nucleoplasm, plasma membrane |
| Calculated MW: | 103kDa |
| Observed MW: | 103kDa |
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
| Purification Method: | Affinity purification |
| Gene ID: | 83737 |
| Clone Number: | ARC1759 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of various cell lines, using ITCH Rabbit mAb (CAB8624) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s. |
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