The Integrin Alpha 6 (ITGA6 / CD49f) Polyclonal Antibody (CAB20807) is a vital tool for researchers studying integrin alpha 6, a cell surface protein involved in cell adhesion and migration. This antibody, produced using rabbits, shows strong reactivity with human samples and has been thoroughly validated for use in Western blot applications. By specifically binding to the ITGA6 protein, researchers can easily detect and analyze its expression in various cell types, making it an excellent choice for studies in cell biology and cancer research.
Integrin alpha 6 serves as a critical mediator in cell-cell and cell-matrix interactions, playing a crucial role in processes such as tissue development, wound healing, and cancer metastasis. Its dysregulation has been implicated in various diseases, making it a promising target for therapeutic interventions. Investigating the function of integrin alpha 6 with the help of this antibody could provide valuable insights into its role in health and disease, paving the way for the development of targeted therapies in the future.
Cell membrane, Lipid-anchor, Single-pass type I membrane protein.
Calculated MW:
127kDa
Observed MW:
125kDa
The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 6 subunit. This subunit may associate with a beta 1 or beta 4 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. The alpha 6 beta 4 integrin may promote tumorigenesis, while the alpha 6 beta 1 integrin may negatively regulate erbB2/HER2 signaling. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3655
Clone Number:
ARC51524
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Integrin alpha 6 (ITGA6/CD49f) antibody (CAB20807) at1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
