The Anti-iNOS Antibody (CAB3774) is a crucial tool for researchers studying inducible nitric oxide synthase (iNOS), an enzyme involved in the immune response and inflammation. This polyclonal antibody, generated in rabbits, shows high reactivity with human iNOS and has been validated for Western blot applications.iNOS is a key player in the production of nitric oxide (NO), a signaling molecule that plays a crucial role in various physiological processes, including host defense, inflammation, and vasodilation. Dysregulation of iNOS and NO production has been implicated in numerous diseases, including cancer, autoimmune disorders, and infectious diseases.
By targeting and detecting iNOS protein, researchers can gain valuable insights into the role of this enzyme in disease pathology and potentially develop new therapeutic strategies to modulate iNOS activity. The Anti-iNOS Antibody (CAB3774) is an essential tool for any study investigating the complex and critical role of iNOS in immune responses and disease progression.
Product Name:
iNOS Rabbit Monoclonal Antibody
SKU:
CAB3774
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 2-200 of human iNOS (P35228).
cortical cytoskeleton, cytoplasm, cytosol, nucleoplasm, nucleus, perinuclear region of cytoplasm, peroxisomal matrix, peroxisome, plasma membrane
Calculated MW:
131kDa
Observed MW:
130kDa
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Purification Method:
Affinity purification
Gene ID:
4843
Clone Number:
ARC0832
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from RAW264.7 cells, using iNOS Rabbit mAb (CAB3774) at 1:1000 dilution.RAW264.7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
