The Anti-IL2 Antibody (CAB20224) is a highly specific and reliable tool for research involving IL2, a cytokine that plays a crucial role in the regulation of immune responses. This polyclonal antibody, raised in rabbits, is optimized for detection and analysis of IL2 protein in human samples, making it ideal for use in Western blot applications. By targeting IL2, researchers can gain valuable insights into the mechanisms underlying immune regulation and potential therapeutic targets for various diseases.IL2, also known as interleukin-2, is a key player in the activation and proliferation of T cells, making it essential for proper immune function.
Dysregulation of IL2 signaling has been implicated in autoimmune diseases, inflammatory disorders, and cancer, highlighting the importance of studying this cytokine in various research contexts. The Anti-IL2 Antibody allows for precise detection and characterization of IL2 protein levels, providing researchers with a valuable tool for advancing our understanding of immune responses and disease pathogenesis.
Product Name:
IL2 Rabbit Monoclonal Antibody
SKU:
CAB20224
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human IL2 (NP_000577.2).
Recombinant Human IL-2 Protein,Jurkat treated by TPA,A23187 and Brefeldin A
Conjugate:
Unconjugated
Cellular Localization:
Secreted.
Calculated MW:
18kDa
Observed MW:
14kDa/15kDa/16kDa/18kDa
This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Purification Method:
Affinity purification
Gene ID:
3558
Clone Number:
ARC5130-01
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using IL2 Rabbit mAb (CAB20224) at 1:1000 dilution.Jurkat cells were treated by TPA (40 nM),A23187 (2μM) and Brefeldin A (300 ng / ml) for 0-24hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
