IL2 Rabbit Monoclonal Antibody (CAB20224)
- SKU:
- CAB20224
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- IL2
- IL-2
- TCGF
- lymphokine
- Research Area:
- Immunology
Description
| Product Name: | IL2 Rabbit Monoclonal Antibody |
| SKU: | CAB20224 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human IL2 (NP_000577.2). |
| Sequence: | MYRM QLLS CIAL SLAL VTNS APTS SSTK KTQL QLEH LLLD LQMI LNGI NNYK NPKL TRML TFKF YMPK KATE LKHL QCLE EELK PLEE VLNL AQSK NFHL RPRD LISN INVI VLEL KGSE TTFM CEYA DETA TIVE FLNR WITF CQSI ISTL T |
| Tested Applications: | WB FC ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 FC,1:50 - 1:200 |
| Synonyms: | IL-2; TCGF; lymphokine; IL2 |
| Positive Sample: | Recombinant Human IL-2 Protein,Jurkat treated by TPA,A23187 and Brefeldin A |
| Conjugate: | Unconjugated |
| Cellular Localization: | Secreted. |
| Calculated MW: | 18kDa |
| Observed MW: | 14kDa/15kDa/16kDa/18kDa |
This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
| Purification Method: | Affinity purification |
| Gene ID: | 3558 |
| Clone Number: | ARC5130-01 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from Jurkat cells, using IL2 Rabbit mAb (CAB20224) at 1:1000 dilution.Jurkat cells were treated by TPA (40 nM),A23187 (2μM) and Brefeldin A (300 ng / ml) for 0-24hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s. |
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