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IL10 Rabbit Monoclonal Antibody (CAB12255)

SKU:
CAB12255
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
CSIF
GVHDS
IL-10
IL10A
TGIF
Research Area:
Cell Biology
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:IL10 Rabbit Monoclonal Antibody
SKU:CAB12255
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IL10 (P22301).
Sequence:MHSS ALLC CLVL LTGV RASP GQGT QSEN SCTH FPGN LPNM LRDL RDAF SRVK TFFQ MKDQ LDNL LLKE SLLE DFKG YLGC QALS EMIQ FYLE EVMP QAEN
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:CSIF; TGIF; GVHDS; IL-10; IL10A; IL10
Positive Sample:THP-1,Mouse spleen,Rat thymus,HeLa
Conjugate:Unconjugated
Cellular Localization:extracellular region, extracellular space
Calculated MW:21kDa
Observed MW:18kDa

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Purification Method:Affinity purification
Gene ID:3586
Clone Number:ARC0650
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using IL10 Rabbit mAb (CAB12255) at 1:1000 dilution.THP-1 cells were treated by LPS and Brefeldin A for different times of stimulation.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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