The Ikzf3 Polyclonal Antibody (CAB8614) is a valuable tool for researchers studying Ikzf3, a transcription factor involved in immune cell development and function. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to Ikzf3 protein, this antibody enables precise detection and analysis in a variety of cell types, making it well-suited for investigations in immunology and cancer research.Ikzf3, also known as Aiolos, plays a crucial role in the regulation of immune responses by controlling the differentiation and function of various immune cell populations.
Its involvement in immune cell development and function positions it as a key target for studies related to autoimmune disorders, cancer, and inflammatory conditions. Comprehensive understanding of Ikzf3 function is essential for the development of therapies aimed at modulating immune responses in these disease contexts.Overall, the Ikzf3 Polyclonal Antibody (CAB8614) serves as a valuable tool for researchers seeking to explore the intricate mechanisms of immune regulation and cell function mediated by Ikzf3, ultimately contributing to advancements in the fields of immunology and cancer research.
Product Name:
IKZF3 Rabbit Monoclonal Antibody
SKU:
CAB8614
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 400-509 of human IKZF3 (Q9UKT9).
WB,1:100 - 1:500 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
AIO; IMD84; AIOLOS; ZNFN1A3; IKZF3
Positive Sample:
Raji,Mouse spleen,Mouse thymus,Rat lung
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Cytosol, Nucleoplasm, Nucleus
Calculated MW:
58kDa
Observed MW:
65kDa
This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described, as well as some non-protein coding variants.
Purification Method:
Affinity purification
Gene ID:
22806
Clone Number:
ARC2781
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Raji cells, using IKZF3 Rabbit mAb (CAB8614) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
