The IKBalpha Polyclonal Antibody (CAB11397) is a crucial tool for studying IKBalpha, a key regulator of the NF-kappa-B pathway involved in immune and inflammatory responses. This antibody, validated for knockout (KO) studies, is raised in rabbits and exhibits high reactivity with human samples, making it ideal for Western blot applications.IKBalpha is a pivotal player in controlling NF-kappa-B activation, a signaling pathway that regulates genes involved in immune and inflammatory responses. Dysregulation of this pathway has been implicated in various diseases, including cancer, autoimmune disorders, and inflammatory conditions.
By targeting and studying IKBalpha, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic strategies. Overall, the IKBalpha Polyclonal Antibody is an essential tool for researchers in immunology, oncology, and inflammation studies, enabling precise detection and analysis of IKBalpha protein levels in a variety of cell types. Its validation for KO studies adds an extra layer of reliability, making it a valuable asset for any research involving the NF-kappa-B pathway.
Antibody Name:
Anti-IkBAlpha Antibody [KO Validated]
Antibody SKU:
CAB11397
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IP
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human I?BAlpha (NP_065390.1).
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
UniProt Protein Function:
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
NCBI Summary:
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3
Protein Family:
NF-kappa-B inhibitor
UniProt Gene Name:
NFKBIAÂ Â
UniProt Entry Name:
IKBA_HUMAN
Western blot analysis of extracts of various cell lines, using IkBalpha antibody (CAB11397) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and IkBalpha knockout (KO) 293T cells, using IkBalpha antibody (CAB11397) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug IkBalpha antibody (CAB11397). Western blot was performed from the immunoprecipitate using IkBalpha antibody (CAB11397) at a dilition of 1:500.
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