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Human IgM Rabbit Polyclonal Antibody (CAB8677)

SKU:
CAB8677
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
IGHM
AGM1
MU
VH
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human IgM Rabbit Polyclonal Antibody
SKU:CAB8677
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 213-453 of human IGHM (P01871).
Sequence:MCVP DQDT AIRV FAIP PSFA SIFL TKST KLTC LVTD LTTY DSVT ISWT RQNG EAVK THTN ISES HPNA TFSA VGEA SICE DDWN SGER FTCT VTHT DLPS PLKQ TISR PKGV ALHR PDVY LLPP AREQ LNLR ESAT ITCL VTGF SPAD VFVQ WMQR GQPL SPEK YVTS APMP EPQA PGRY FAHS ILTV SEEE WNTG ETYT CVVA HEAL PNRV TERT VDKS TGKP TLYN VSLV MSDT AGTC Y
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:MU; VH; AGM1; Human IgM
Positive Sample:Raji
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:49kDa
Observed MW:80kDa

Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).

Purification Method:Affinity purification
Gene ID:3507
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Raji cells, using Human IgM Rabbit pAb (CAB8677) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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