The Anti-Human IgM Antibody (CAB19719) is a valuable tool for research involving IgM, an important component of the immune system responsible for the initial response to infections. This antibody, raised in rabbits, is highly specific for human IgM and has been validated for use in various applications including Western blot, ELISA, and immunohistochemistry.IgM is the first antibody produced in response to an infection, and its detection is crucial for understanding the body's immune response. This antibody binds specifically to IgM proteins, allowing for sensitive and accurate detection in a variety of sample types.
It is ideal for studies in immunology, infectious diseases, and autoimmune disorders where IgM levels play a critical role in disease progression and diagnosis.With its high reactivity and specificity for human IgM, the Anti-Human IgM Antibody (CAB19719) is a valuable tool for researchers looking to explore the role of IgM in immune responses and disease pathology. Its versatility in different assay formats makes it a valuable asset for laboratories investigating a wide range of diseases and conditions related to the immune system.
Product Name:
Human IgM Rabbit Monoclonal Antibody
SKU:
CAB19719
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-453 of human IgM (P01871).
blood microparticle, cell surface, external side of plasma membrane, extracellular exosome, extracellular space, immunoglobulin complex, circulating, plasma membrane
Calculated MW:
49kDa
Observed MW:
75kDa
Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).
Purification Method:
Affinity purification
Gene ID:
3507
Clone Number:
ARC2245
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Human plasma, using Human IgM Rabbit mAb (CAB19719) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
