The Anti-Human CD8a FITC / CD4 PE / CD3 PE-Cyanine7 / CD45 PerCP Cocktail from Assay Genie is a comprehensive cellular analysis tool designed for flow cytometry applications in immunology and cell biology research. This cocktail combines multiple fluorescent antibodies specific to key cell surface markers - CD8a, CD4, CD3, and CD45 - allowing for simultaneous identification and characterization of different immune cell populations within human samples.The CD8a FITC antibody targets cytotoxic T cells, CD4 PE antibody binds to helper T cells, CD3 PE-Cyanine7 antibody recognizes T cell receptor complex, and CD45 PerCP antibody identifies all leukocytes. By using this cocktail, researchers can gain detailed insights into the composition and activation status of T cell subsets, aiding in the study of immune responses, immune cell interactions, and disease mechanisms.
With high reactivity and specificity to human samples, this antibody cocktail offers reliable and reproducible results for flow cytometry experiments. It is an invaluable tool for investigating immune system function, monitoring immune cell populations in health and disease, and exploring potential therapeutic targets for conditions such as infections, autoimmune disorders, and cancer. Upgrade your research capabilities with the Anti-Human CD8a FITC / CD4 PE / CD3 PE-Cyanine7 / CD45 PerCP Cocktail from Assay Genie.
For whole blood samples, add 5 μL Anti-Human CD8a-FITC/CD4-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail to 100 μL anticoagulant-treated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection. For other samples, 1×106 dissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 μL cell staining buffer and add 5 μL Anti-Human CD8a- FITC/CD4-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.
Spectrum:
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
This product is a FCM antibody cocktail made up of FITC Anti-Human CD8a Antibody [Clone: OKT-8] (Mouse IgG2a, κ), PE Anti-Human CD4 Antibody [Clone: RPA-T4] (Mouse IgG1, κ), PE/Cyanine7 Anti-Human CD3 Antibody [Clone: OKT-3] (Mouse IgG2a, κ) and PerCP Anti-Human CD45 Antibody [Clone: HI30] (Mouse IgG1, κ).; CD8 is mainly expressed on cytotoxic T cells and also some subpopulations of NK cells. CD8 forms dimer function. In most cells, CD8 is a heterodimer consisting of CD8a and CD8b, but in NK cells nearly all CD8 is homodimer of CD8a. CD8a can form co-receptor with MHC-I restricted TCR to promote T cell antigen recognition and activation.; CD4 is also called Leu-3 or T4. It's a single-chain type I transmembrane glycoprotein, mainly expressed on the surface of T cells, and monocytes/macrophages. In T cells, CD4 forms complex with TCR/CD3 and play important roles in T cell immunity. The target of HIV is CD4+ T cells. Reduction of CD4+ T cells is the main reason of defected immunity after HIV infection.; CD3 is a heterotetrameric protein consisting of a CD3γ, a CDδ and 2 CD3ε. It forms complex with TCR. OKT-3 recognize human CD3ε. Human CD3 is expressed on the surface of T cells and NKT cells.; CD45 is a single-chain type I transmembrane glycoprotein. Except for erythrocytes and platelets, CD45 is expressed on nearly all of the hematopoietic cells with high level. It is a common marker for blood leukocytes. CD45 is a receptor type protein tyrosine phophatase and plays essential roles in B cell and T cells signaling.
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