Anti-Human CD68 In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Human CD68 In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody tailored for in vivo applications. Targeting the CD68 protein, a key player in the immune response, this antibody is essential for research in immunology, oncology, and related disciplines. Featuring a mouse IgG2a isotype, it guarantees high purity and ultra-low endotoxin levels (<0.01 EU/μg), making it optimal for ELISA, flow cytometry, immunohistochemistry, and various other assays. The antibody is available in different sizes and is meticulously formulated in phosphate-buffered saline to ensure stability and efficacy throughout your experiments.
Elevate your research with this dependable and adaptable antibody from Assay Genie.
CD68 is a glycoprotein predominantly expressed on the surface of monocytes and macrophages, which are vital components of the immune system. CD68 is implicated in phagocytic activities and serves as a macrophage marker, aiding in the clearance of cellular debris and pathogens. It plays a significant role in various immune processes, thereby making it a critical marker for studying inflammatory responses, tumor microenvironments, and macrophage-related diseases.
Product Name:
Anti-Human CD68 In Vivo Antibody - Ultra Low Endotoxin
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Anti-CD68 antibody (clone KP1) activity is directed against human CD68.
Antigen Distribution:
CD68 is found in the cytoplasm of monocytes/macrophages, fibroblasts, human peripheral blood lymphocytes, neutrophil primary and mast cell granules, large granular lymphocytes, basophils, basal epithelial cell layers, renal glomeruli, myeloid cells, endothelial cells, retinal epithelial cells, osteoblasts, fibroblast-like cells from bone marrow, and a wide-range of lymphoid neoplasms. CD68 is predominantly located in lysosomal membranes, with a small amount on the cell surface. Additionally, CD68 can be expressed in most hematopoietic cell lines by phorbol-induced differentiation; soluble CD68 can also be found in serum and urine.
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
CD68 is a heavily glycosylated type I transmembrane glycoprotein belonging to the lysosomal-associated membrane protein (LAMP) family and may play a role in endocytosis or lysosomal traffic and phagocytic activities1. CD68 localizes to endosome- or lysosome-like structures2. Additionally, LAMPS have been implicated in tumor cell metastasis and are over-expressed on the surface of metastatic compared with nonmetastatic cell lines3.
KP1 was originally used as a pan-monocytic/macrophage marker against CD68; however, CD68 is not a macrophage specific antigen and KP1 is known to stain neutrophils and other non-macrophage-like cells4,5,6,7,8. KP1 reacts against CD68 in a wide range of healthy4 and disease-associated (rheumatoid arthritis and osteoarthritis)5 tissues as well as a variety of neoplasms9, tumor cell lines7, and tumor-associated macrophages 8. KP1 detects a fixation-resistant epitope10 that is likely glycan-based1,9 which is shared by many cell types7.
KP1 was developed by immunizing Balb/c mice against a lysosomal fraction of human lung 10. Hybridoma supernatants derived from spleen cells were screened on cryostat human lung and tonsil sections as well as paraffin wax sections of lung tissue fixed in formol saline.
Research Area:
Immunology
Pathogen Testing:
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
