Anti-Human CD56 (NCAM) [ERIC-1] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human CD56 [NCAM] [Eric-1] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD56 protein, also known as Neural Cell Adhesion Molecule (NCAM), which is crucial for cell adhesion and communication, making it ideal for research in neurobiology, immunology, and related fields. With a mouse IgG1 isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for applications such as ELISA, flow cytometry, immunoprecipitation, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody.
CD56 (NCAM) is a glycoprotein expressed on the surface of various cell types, including natural killer (NK) cells, a subset of T-cells, and neural tissues. It plays a significant role in immune response and neural connectivity by mediating cell-cell interactions and signal transduction. This makes it a valuable marker in studies related to the nervous system function, development, and immune system disorders.
Product Name:
Anti-Human CD56 (NCAM) (Clone ERIC-1) In Vivo Antibody - Low Endotoxin
Product Code:
IVMB0339
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
ERIC-1
Protein:
CD56
Product Type:
Monoclonal Antibody
Synonyms:
NCAM, Neural cell adhesion molecule, NKH1, Leu-19
Isotype:
IgG1
Reactivity:
Human
Applications:
Dot, ELISA Cap, IHC FFPE, In Vivo, PhenoCycler®, WB
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Purity:
>95% by SDS-PAGE and HPLC
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
Dot, ELISA Cap, IHC FFPE, In Vivo, PhenoCycler®, WB
Reactivity:
Human
Host Species:
Mouse
Specificity:
Mouse Anti-Human CD56 (Clone ERIC-1) recognizes Human CD56. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD56 recognizes the (Mr 180, 145, 125 kDa) isoforms of the human neural cell adhesion molecule (NCAM).1
Antigen Distribution:
The CD56 (NCAM) antigen is expressed on most neuroectodermal derived cell lines, tissues, and tumors. NCAM is also known to be expressed on some mesodermally derived tissues such as muscle1 and on natural killer (NK) lymphocytes. The binding of ERIC-1 to both normal and neoplastic tissue is lost when tissues are conventionally fixed in formalin and embedded in paraffin. The epitope was preserved when exposed to dehydrating fixatives such as cold acetone (-20EC) for 5 min. CD56 is present on 10-25% of peripheral blood lymphocytes.
Immunogen:
Human Retinoblastoma tumor
Concentration:
1.0 - 5.0 mg/ml
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
>95% by SDS-PAGE and HPLC
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Anti-Human CD56 can be used in studies of the neural cell adhesion molecule. This antibody may also be used to study isoforms of NCAM expressed on different tumor types. Anti-CD56 (clone ERIC-1) works well in western blot for analysis of isoforms expressed. Anti-CD56 did not react with any of the leukemias or lymphomas tested.1
