Anti-Human CD45 [BC8] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human CD45 [BC8] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody meticulously engineered for in vivo applications. This antibody targets the CD45 protein, a critical regulator of antigen receptor signaling in lymphocytes, making it an excellent tool for research in immunology and related fields. Featuring a mouse IgG1 isotype, it guarantees high purity and remarkably low endotoxin levels (<1.0 EU/mg), ensuring optimal performance in ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various quantities, it is formulated in phosphate-buffered saline to maintain stability and maximize efficacy.
Enhance your research endeavors with this reliable and versatile antibody. CD45, also known as the leukocyte common antigen (LCA), is a protein tyrosine phosphatase located on the surface of almost all nucleated cells within the hematopoietic system, including T cells, B cells, and myeloid cells. It plays a pivotal role in regulating and facilitating signal transduction pathways essential for lymphocyte activation, development, and function. By targeting CD45, this antibody aids in advancing our understanding of immune cell behavior and immune-related responses.
Product Name:
Anti-Human CD45 (Clone BC8) In Vivo Antibody - Low Endotoxin
Product Code:
IVMB0346
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
BC8
Protein:
CD45
Product Type:
Monoclonal Antibody
Synonyms:
PTPRC
Isotype:
IgG1
Reactivity:
Human
Applications:
FA, In Vivo
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Purity:
≥95% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
FA, In Vivo
Reactivity:
Human
Host Species:
Mouse
Specificity:
Clone BC8 recognizes an epitope on human CD45.
Antigen Distribution:
CD45 is expressed on all hematopoietic cells other than mature erythrocytes and platelets.
Concentration:
2.0 mg/ml
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥95% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase supression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A, B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform.
