The Anti-Human CD3 FITC, CD4 PE, CD8a PE-Cyanine7, CD45 PerCP Cocktail from Assay Genie is a comprehensive set of antibodies designed for immunophenotyping and multi-color flow cytometry analysis of human immune cell subsets. This cocktail combines four different fluorochrome-conjugated antibodies targeting CD3, CD4, CD8a, and CD45 antigens, allowing for simultaneous detection and characterization of T cell populations.CD3 is a pan T cell marker, CD4 and CD8a are cell surface markers found on T helper and cytotoxic T cells respectively, while CD45 is a common leukocyte antigen essential for identifying hematopoietic cells. By utilizing this cocktail, researchers can easily distinguish between different T cell subsets and leukocyte populations in various immune-related studies.
This antibody cocktail provides high specificity and sensitivity in detecting these critical cell surface markers, enabling precise analysis of immune cell composition and function. It is an invaluable tool for studying immune responses, immune cell profiling, and monitoring changes in immune cell populations under different conditions, making it ideal for research in immunology, infectious diseases, autoimmune disorders, and immunotherapy development.Overall, the Anti-Human CD3 FITC, CD4 PE, CD8a PE-Cyanine7, CD45 PerCP Cocktail offers a reliable and efficient solution for researchers seeking to explore the dynamics of human immune cell subpopulations and their roles in health and disease.
For whole blood samples, add 5 μL Anti-Human CD3-APC/CD4-FITC/CD8a-PE Cocktail to 100 μL anticoagulant- treated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection. For other samples, 1×106 dissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 μL cell staining buffer and add 5 μL Anti-Human CD3-APC/CD4-FITC/CD8a-PE Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.
Spectrum:
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
This product is a FCM antibody cocktail made up of FITC Anti-Human CD3 Antibody [Clone: OKT-3] (Mouse IgG2a, κ),PE Anti-Human CD4 Antibody [Clone: RPA-T4] (Mouse IgG1, κ), PE/Cyanine7 Anti-Human CD8a Antibody[Clone: OKT-8] (Mouse IgG2a, κ) and PerCP Anti-Human CD45 Antibody [Clone: HI30] (Mouse IgG1, κ).; CD3 is a heterotetrameric protein consisting of a CD3γ, a CDδ and 2 CD3ε. It forms complex with TCR. OKT-3 recognize human CD3ε. Human CD3 is expressed on the surface of T cells and NKT cells.; CD4 is also called Leu-3 or T4. It's a single-chain type I transmembrane glycoprotein, mainly expressed on the surface of T cells, and monocytes/macrophages. In T cells, CD4 forms complex with TCR/CD3 and play important roles in T cell immunity. The target of HIV is CD4+ T cells. Reduction of CD4+ T cells is the main reason of defected immunity after HIV infection.; CD8 is mainly expressed on cytotoxic T cells and also some subpopulations of NK cells. CD8 forms dimer function. In most cells, CD8 is a heterodimer consisting of CD8a and CD8b, but in NK cells nearly all CD8 is homodimer of CD8a. CD8a can form co-receptor with MHC-I restricted TCR to promote T cell antigen recognition and activation.; CD45 is a single-chain type I transmembrane glycoprotein. Except for erythrocytes and platelets, CD45 is expressed on nearly all of the hematopoietic cells with high level. It is a common marker for blood leukocytes. CD45 is a receptor type protein tyrosine phophatase and plays essential roles in B cell and T cells signaling.
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