The Anti-Human CD3 FITC/CD19 PE Cocktail from Assay Genie is a powerful tool for immunophenotyping studies involving human T cells and B cells. This ready-to-use cocktail contains fluorescently labeled antibodies targeting CD3 and CD19, two important cell surface markers for identifying T lymphocytes and B lymphocytes, respectively.The CD3 FITC antibody specifically binds to the CD3 protein complex found on T cells, while the CD19 PE antibody targets the CD19 antigen expressed on B cells. By utilizing this cocktail, researchers can easily distinguish and quantify T cells and B cells in a mixed population, enabling detailed analysis of immune cell subsets in various experimental settings.With its high specificity and sensitivity, the Anti-Human CD3 FITC/CD19 PE Cocktail is ideal for flow cytometry applications, such as immunophenotyping studies, immune monitoring, and cell subset analysis.
This versatile reagent streamlines the process of characterizing immune cell populations, making it a valuable asset for immunology research, drug development, and clinical diagnostics.Whether investigating immune responses in disease models, studying immune cell dynamics in cancer immunotherapy, or evaluating the efficacy of immunomodulatory treatments, the Anti-Human CD3 FITC/CD19 PE Cocktail offers precise and reliable detection of T cells and B cells, facilitating in-depth exploration of immune system function and dysregulation. Advance your immunology research with this innovative cocktail from Assay Genie.
Product Title:
Anti-Human CD3-FITC/CD19-PE Cocktail
SKU:
AGEL2712
Size:
20 Tests, 100 Tests, 200 Tests
Clone No:
OKT-3 , CB19 ,
Reactivity:
Human
Application:
FCM
Form:
Liquid
Conjugation:
FITC;PE;
Recommended Use:
For whole blood samples, add 5 μL Anti-Human CD3-FITC/CD19-PE Cocktail to 100 μL anticoagulant-treated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection. For other samples, 1×106 dissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 μL cell staining buffer and add 5 μL Anti-Human CD3-FITC/CD19-PE Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.
Spectrum:
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
This product is a FCM antibody cocktail made up of FITC Anti-Human CD3 Antibody [Clone: OKT-3] (Mouse IgG2a, κ) and PE Anti-Human CD19 Antibody [Clone: CB19] (Mouse IgG1, κ).; CD3 is a heterotetrameric protein consisting of a CD3γ, a CDδ and 2 CD3ε. It forms complex with TCR. OKT-3 recognize human CD3ε. Human CD3 is expressed on the surface of T cells and NKT cells.; CD19 is a single-chain transmembrane glycoprotein expressed on B cells of all stages except plasma cells. It is a common marker for B cells. CD19 is also expressed in follicular dendritic cells. It forms complex with CD21 and CD84, which forms co-receptor with BCR. It takes part in B cell development, activantion and differentiation.
![FITC Anti-Human CD19 Antibody [CB19]](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/229899/605244/fitc-anti-human-cd19-antibody-cb19__68955.1706889779.jpg?c=2 "FITC Anti-Human CD19 Antibody [CB19]")