Introducing the Anti-Human CD28 In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD28 protein, a critical co-stimulatory molecule involved in T-cell activation and survival, making it indispensable for research in immunology and related fields.
With a mouse IgG1 isotype, it provides high purity and low endotoxin levels (<1.0 EU/mg), ensuring optimal performance in ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for enhanced stability and efficacy. Elevate your research with this reliable and versatile antibody.
CD28 is a surface protein expressed on T-cells, which plays a crucial role in the immune system by providing co-stimulatory signals necessary for T-cell proliferation, cytokine production, and survival. Its interaction with ligands B7-1 (CD80) and B7-2 (CD86) on antigen-presenting cells (APCs) is vital for effective immune responses, making it a key focus in immunological research and therapeutic development.
Product Name:
Anti-Human CD28 In Vivo Antibody - Low Endotoxin
Product Code:
IVMB0325
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
CD28.2
Protein:
CD28
Product Type:
Monoclonal Antibody
Synonyms:
MGC138290, Tp44
Isotype:
IgG1 k
Reactivity:
Human
Applications:
Costim, FC, IHC FF, In Vivo, IP, PhenoCycler®
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Purity:
≥95% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
Costim, FC, IHC FF, In Vivo, IP, PhenoCycler®
Reactivity:
Human
Host Species:
Mouse
Specificity:
Clone CD28.2 recognizes an epitope on human CD28.
Antigen Distribution:
CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells.
Concentration:
≥ 2.0 mg/ml
Endotoxin Level:
< 1.0 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥95% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.
