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Anti-Human CD20 (Rituximab) - PE Biosimilar (IVMB0525)

SKU:
IVMB0525
Antibody Type:
PE Biosimilar
Modifications:
PE-Conjugated
Applications:
FC
Drug Development
Inflammatory Response Assays
Pharmacokinetics
Disease Area:
B-Cell Lymphomas
Disease Area:
Autoimmune Diseases
$551

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Anti-Human CD20 (Rituximab) - PE
SKU:IVMB0525
Size:50 µg
Antibody Type:Biosimilar Recombinant Human Monoclonal Antibody
Clone:10F381
Target:CD20
Isotype:Human IgG1κ
Host Species:Human
Reactivity:Human/Rhesus Monkey/Cynomolgus Monkey
Applications:FC
Expression Host:HEK-293 Cells
FC Effector Activity:Active
Synonyms:B1; S7; Bp35; CVID5; MS4A2; LEU-16; MS4A1; membrane spanning 4-domains A1
Product Concentration:0.2 mg/ml
Excitation Laser:Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Immunogen:Human lymphoblastoid cell line SB.
Applications and Recommended Usage:FC The suggested concentration for Rituximab biosimilar antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Antigen Distribution:CD20 is primarily found on the surface of immune system B cells. CD20 is highly expressed in the lymph node, and to a lesser extent, the spleen and appendix.
Formulation:This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Specificity:This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Rituximab. Clone 10F381 recognizes human CD20. This product is for research use only.

CD20 is a 33-37 kD transmembrane-spanning phosphoprotein found on the surface of developing B-cells and various B-cell malignancies. CD20 is a popular target for mAb therapy because depleting developing B-cells generally does not cause permanent side effects (due to the fact that mature plasma cells and B-cell progenitors do not express CD20 and that there is limited expression of CD20 among other cell lineages). Rituximab is a chimeric monoclonal antibody that binds to CD20. The precise function of CD20 is still unknown. However, it is suspected to play a role in Ca2+ influx across plasma membranes, maintaining intracellular Ca2+ concentration, and allowing the activation of B cells. Rituximab is used to treat some autoimmune diseases and types of cancer such as non-Hodgkin lymphoma, chronic lymphocytic leukemia, and rheumatoid arthritis among others. The Fc portion of Rituximab mediates antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). Rituximab increases MHC II and adhesion molecules LFA-1 and LFA-3 (lymphocyte function-associated antigen) and also induces apoptosis of CD20+ cells. This ultimately results in the elimination of B cells (including the cancerous ones) from the body, and thus allows a new population of healthy B cells to develop from lymphoid stem cells. Anti-Human CD20 (Rituximab) utilizes the same variable regions from the therapeutic antibody Rituximab making it ideal for research projects.

Regulatory Status:Research Use Only (RUO). Non-Therapeutic.
Storage and Handling:This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C.Do not freeze.
Research Area:Biosimilars

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