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Anti-Human CD20 (Obinutuzumab) - PE Biosimilar (IVMB0528)

SKU:
IVMB0528
Antibody Type:
PE Biosimilar
Modifications:
PE-Conjugated
Applications:
FC
Drug Development
Inflammatory Response Assays
Pharmacokinetics
Disease Area:
B-Cell Lymphomas
Disease Area:
Autoimmune Diseases
€499

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Anti-Human CD20 (Obinutuzumab) - PE
SKU:IVMB0528
Size:50 µg
Antibody Type:Biosimilar Recombinant Human Monoclonal Antibody
Clone:GA101
Target:CD20
Isotype:Human IgG1κ
Host Species:Human
Reactivity:Human
Applications:ELISA
Expression Host:HEK-293 Cells
Synonyms:Obinutuzumab, CD20, MS4A1
Product Concentration:0.2 mg/ml
Excitation Laser:Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Immunogen:Human lymphoblastoid cell line SB.
Applications and Recommended Usage:FC,
ELISA
Antigen Distribution:CD20 is a general B cell marker expressed by the majority of normal B cells in all stages of their development as well as by most B cell malignancies.
Formulation:This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Specificity:Obinutuzumab (GA101) activity is directed against human CD20.

CD20 is a nonglycosylated 33-37 kDa phosphoprotein member of the MS4A family which is widely expressed on normal B cell surfaces during all stages of development as well as by most B cell malignancies1,2. The biological role of CD20 remains poorly understood; however, it is thought to be involved in calcium ion influx. CD20 has no natural ligand and is not immediately internalized upon antibody binding. Thus, mAbs directed against CD20 depend on the recruitment of a host response. Anti-CD20 mAbs bind to the 44 amino acid extracellular portion.

Obinutuzumab (GA101) is a new generation, type II, anti-CD20 antibody2. Obinutuzumab was humanized by grafting the complementarity-determining sequences of murine IgG1-κ antibody B-Ly1 onto human VH and VL acceptor frameworks3. The Fc segment was glycoengineered to attach bisected, complex, nonfucosylated oligosaccharides to asparagine 297, leading to increased affinity to FcgRIII.

Obinutuzumab causes homotypic adhesion4,5,6, induces direct cell death via largely caspase-independent mechanisms4,6,7,8,9, does not localize into lipid rafts4,10,11, displays half-maximal CD20 binding at saturating conditions7, and displays minimal complement dependent cytotoxicity7.

Compared to rituximab, obinutuzumab recognizes a distinct but overlapping CD20 epitope, in a different orientation that results in increased pro-apoptotic potential12,13,14. A modified elbow-hinge residue, characterized by a leucine to valine mutation at Kabat position 11, is key to superior phosphatidylserine exposure and cell death relative to rituximab3.

Ligand/Receptor:Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
State of Matter:Liquid
Regulatory Status:Research Use Only (RUO). Non-Therapeutic.
Storage and Handling:This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C.Do not freeze.
UniProt:P11836
Research Area:Biosimilars

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