Anti-Human CD195 (CCR5) In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Human CD195 (CCR5) In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody optimized for in vivo applications. This antibody targets the CD195 (CCR5) protein, a critical chemokine receptor involved in immune responses and HIV infection, making it indispensable for research in immunology, virology, and related fields. With its mouse IgG2a kappa isotype, this antibody guarantees high purity and ultra-low endotoxin levels (<0.01 EU/μg), suitable for ELISA, flow cytometry, immunohistochemistry, and other sensitive assays. It is available in various sizes and formulated in phosphate-buffered saline to ensure stability and efficacy.
Elevate your research with this reliable and versatile antibody. CD195 (CCR5) is a G-protein-coupled receptor found on the surface of immune cells such as T cells, macrophages, dendritic cells, and certain subsets of lymphocytes. It plays a significant role in the immune system by mediating cell trafficking and signaling in response to chemokines, and it acts as a coreceptor for HIV entry into host cells.
Product Name:
Anti-Human CD195 (CCR5) In Vivo Antibody - Ultra Low Endotoxin
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
≤ 0.5 EU/mg as determined by the LAL method
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
FC, ICC, In Vivo
Reactivity:
Human
Host Species:
Rat
Specificity:
Clone HEK/1/85a/7a recognizes an epitope on human CCR5.
Antigen Distribution:
CCR5 is expressed on memory T cells, macrophages, and immature dendritic cells (DCs).
Immunogen:
CHO cells transfected with full-length human CD195 (CCR5).
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
≤ 0.5 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
CCR5 antibody, HEK/1/85a/7a, recognizes C-C chemokine receptor type 5 (CCR5), also known as CD195, CC-CKR-5, CHEMR13, and HIV-1 fusion coreceptor. CCR5 is a 45 kDa seven-transmembrane G-protein-coupled receptor (GPCR). CCR5 is expressed on a subset of memory T cells, macrophages, and immature dendritic cells (DCs) and is upregulated by proinflammatory cytokines1,2. CCR5 binds to inflammatory CC-chemokines, including CCL3 (macrophage inflammatory protein (MIP)-1α), CCL4 (MIP-1β), and RANTES, which increases intracellular calcium levels and results in cytoskeletal rearrangement and chemotactic cell migration3. CCR5 is also the coreceptor for HIV-1 following binding of viral gp120 to CD44-7. The levels of CCR5 on cells influence the rate of entry of HIV-1, and patients with lower levels of CCR5 exhibit immunity against infection8. CCR5 also contributes to other human diseases, including type I diabetes9 and cancer10.
Protein:
CD195
Pathogen Testing:
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
