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Anti-Human CD11b In Vivo Antibody - Ultra Low Endotoxin

SKU:
IVMB0108
Product Type:
In Vivo Monoclonal Antibody
Clone:
ICRF44
Protein:
CD11b
Isotype:
Mouse IgG1 kappa
Reactivity:
Human
Synonyms:
Mac-1
Integrin alphaM chain
C3biR
CR3
Mo1
ITGAM
Research Area:
Adhesion & Migration
Endotoxin Level:
Ultra Low Endotoxin
Host Species:
Mouse
Applications:
CODEX
FC
IF Staining
IHC FF
$322 - $4,704
Frequently bought together:

Description

system_update_altTechnical Datasheet
Product Name:Anti-Human CD11b In Vivo Antibody - Ultra Low Endotoxin
Product Code:IVMB0108
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:ICRF44
Protein:CD11b
Product Type:Monoclonal Antibody
Synonyms:Mac-1, Integrin αM chain, C3biR, CR3, Mo1, ITGAM
Isotype:Mouse IgG1 κ
Reactivity:Human
Immunogen:Rheumatoid synovial cells and human monocytes.
Applications:FC, IF Staining, IHC FF, PhenoCycler®
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0107Anti-Human CD11b In Vivo Antibody - Low EndotoxinLow
IVMB0108Anti-Human CD11b In Vivo Antibody - Ultra Low EndotoxinUltra Low
Applications:CODEX®, FC, IF Staining, IHC FF
Recommended Usage:FC
The suggested concentration for this ICRF44 antibody for staining cells in flow cytometry is ≤ 1 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:Human
Host Species:Mouse
Specificity:Clone ICRF44 recognizes the α subunit (CD11b) of the human CD11b/CD18 complex.
Antigen Distribution:CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of T-cells and macrophages.
Immunogen:Rheumatoid synovial cells and human monocytes.
Concentration:≥ 5.0 mg/ml
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.

Technical Datasheet:View
Protein:CD11b
Function:Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor:ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Research Area:Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
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