Anti-Human Alpha Defensin-5 (HD5) In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Human Alpha Defensin 5 (HD5) In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the human alpha defensin 5 (HD5), a peptide crucial in the innate immune defense, making it ideal for research in microbiology, immunology, and gastrointestinal studies. With a monoclonal IgG isotype, it ensures high purity and ultra low endotoxin levels (<0.01 EU/μg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays.
Available in various sizes, it is formulated in a phosphate-buffered saline solution for stability and efficacy. Enhance your research with this reliable and versatile antibody.
Human alpha defensin 5 (HD5) is a small antimicrobial peptide primarily found in the granules of Paneth cells in the small intestine. It plays a significant role in the mucosal defense by exerting antimicrobial activity against a broad spectrum of pathogens including bacteria, fungi, and viruses.
Product Name:
Anti-Human Alpha Defensin-5 (HD5) In Vivo Antibody - Ultra Low Endotoxin
Product Code:
IVMB0353
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
8C8
Protein:
HD5
Product Type:
Monoclonal Antibody
Synonyms:
DEFA5; DEF5; HD-5
Isotype:
IgG2b k
Reactivity:
Human
Applications:
Dot, ELISA, IHC FFPE, In Vivo, WB
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
Dot, ELISA, IHC FFPE, In Vivo, WB
Reactivity:
Human
Host Species:
Mouse
Specificity:
Clone 8C8 recognizes human alpha-defensin 5 (HD5).
Antigen Distribution:
HD5 is highly expressed in the secretory granules of Paneth cells of the ileum.
Immunogen:
Recombinant Human HD5 (aa 20-94).
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
The colon lies adjacent to the small intestine and has heavy bacterial colonization from ingested food and water that is often contaminated with bacteria. Remarkably, the small intestine has a low microbial density. A special type of epithelial cell, called a Paneth cell, can be found clustered at the base of the tubular glands that lie between the villi of the inner surface of the small intestine. These cells secrete defensins which have been shown to have activity against both Gram-positive and Gram-negative microbes.3 Six human α-defensins, a subfamily of defensin peptides characterized by their cysteine spacing and disulfide connectivity2, have been identified. Human Paneth cells express α-defensin 5 (HD5) along with HD6. Paneth cells are most numerous in the ileum and have many features similar to those of myeloid cells. They are multifaceted cells with a large quantity of apically-located eosinophilic secretory granules containing lysozyme and other antimicrobial factors which are released upon bacterial stimulation.2 In addition, these cells express tumor necrosis factor alpha (TNF-a), CD1, and CD15.1 Paneth cells do not store defensins as fully processed or active peptides, rather they store them as inactive propeptides.2 They are released as mature peptides after trypsin digestion. Trypsin is also secreted from the Paneth cell granules. It has been reported that disrupted α-defensin processing in murine Paneth cells has initiated a vulnerability to enteric infection.2 HD5 expression levels have been found to be negatively correlated to intestinal infection. Additionally, studies have shown HD5 to be a strong antagonist towards human Papillomavirus infection. Furthermore, low expression of HD5 is thought to play a role in Crohn’s disease.3 Anti-Human Alpha Defensin-5 (HD5) Clone 8C8 has been shown to recognize the propeptide and the partially processed forms of HD5. However, clone 8C8 has been reported to scarcely recognize the mature peptide. There was no reported cross reactivity with HD6, lysozyme, or sPLA2.
Protein:
HD5
Pathogen Testing:
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
![Anti-Human Alpha Defensin-5 [8C8] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214437/569415/anti-human-alpha-defensin-5-8c8-in-vivo-antibody-low-endotoxin__64426.1673496781.jpg?c=2 "Anti-Human Alpha Defensin-5 [8C8] In Vivo Antibody - Low Endotoxin")
