The HNRNP E1 (PCBP1) Polyclonal Antibody (CAB19276) is a valuable tool for researchers studying the role of HNRNP E1 (PCBP1) in various cellular processes. This antibody, developed using rabbits as the host species, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications.HNRNP E1 (PCBP1) is a multifunctional RNA-binding protein involved in regulating gene expression, RNA splicing, and mRNA stability. Its diverse functions make it a key player in cellular processes such as mRNA transport, translation, and post-transcriptional regulation. The HNRNP E1 (PCBP1) antibody binds to the target protein with high specificity, enabling researchers to detect and analyze HNRNP E1 (PCBP1) levels in different cell types.
Understanding the role of HNRNP E1 (PCBP1) is crucial for unraveling its involvement in various physiological and pathological conditions. This antibody is an essential tool for investigating the functions of HNRNP E1 (PCBP1) in areas such as gene regulation, RNA metabolism, and cellular signaling pathways. Researchers studying diseases such as cancer, neurodegenerative disorders, and viral infections will find this antibody invaluable in their research efforts.
Product Name:
hnRNP E1/PCBP1 Rabbit Monoclonal Antibody
SKU:
CAB19276
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PCBP1 (Q15365).
This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
Purification Method:
Affinity purification
Gene ID:
5093
Clone Number:
ARC2456
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using hnRNP E1/hnRNP E1/PCBP1 antibody (CAB19276) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
