The HLA-DRB4 Monoclonal Antibody (CAB0439) is a valuable tool for researchers studying the HLA-DRB4 gene, a member of the human leukocyte antigen (HLA) complex involved in immune system function. This antibody, produced in mice, provides high specificity and sensitivity for detecting HLA-DRB4 in human samples, making it ideal for use in various immunological assays, including Western blotting and immunohistochemistry.HLA-DRB4 is known to play a key role in antigen presentation and immune response regulation, making it a critical target for understanding autoimmune diseases, transplant rejection, and infectious diseases.
The HLA-DRB4 Monoclonal Antibody allows for reliable detection and analysis of HLA-DRB4 expression in different cell types, offering valuable insights into the complex mechanisms of immune system function and disease pathology.By utilizing the HLA-DRB4 Monoclonal Antibody in research studies, scientists can further elucidate the role of HLA-DRB4 in immune system modulation and its implications for various health conditions. This antibody serves as a powerful tool for unraveling the mysteries of the human immune system and exploring potential therapeutic strategies for managing immune-related disorders.
Product Name:
HLA-DRB4 Rabbit Monoclonal Antibody
SKU:
CAB0439
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human HLA-DRB4 (P13762).
Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, Trans-Golgi network membrane
Calculated MW:
30kDa
Observed MW:
30kDa
HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. There are multiple pseudogenes of this gene.
Purification Method:
Affinity purification
Gene ID:
3126
Clone Number:
ARC2505
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of Raji cells, using HLA-DRB4 antibody (CAB0439) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
