The Anti-Histone H4 Antibody (CAB19815) is a valuable tool for researchers studying histone H4, a crucial protein involved in chromatin structure and gene regulation. This antibody, produced in rabbits, exhibits high reactivity with samples from various species and has been validated for use in Western blot and immunofluorescence applications.Histone H4 is a key component of nucleosomes, which are the building blocks of chromatin and play a fundamental role in DNA packaging. Aberrant histone H4 function has been linked to various diseases, including cancer, making it a relevant target for investigation in cancer research and epigenetics studies.
By specifically binding to histone H4, this antibody enables precise detection and analysis of histone H4 levels and modifications in different cell types and experimental conditions. Researchers interested in understanding the role of histone H4 in gene expression, chromatin remodeling, and disease development will find this antibody to be a valuable asset in their research endeavors.
Product Name:
Histone H4 Rabbit Monoclonal Antibody
SKU:
CAB19815
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 24-103 of human Histone H4 (P62805).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
Purification Method:
Affinity purification
Gene ID:
8359
Clone Number:
ARC2340
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Histone H4 Rabbit mAb (CAB19815) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 10s.
